Indirect Genetic Characterization of Serratia marcescens

Abstract

The pure culture isolate of Serratia marcescens, obtained from a clinical specimen was maintained in nutrient agar medium at 4°C. It was genetically characterized by performing 16s rRNA sequence analysis, neighbour tree exposition, phylogenetic tree construction and peptide mass fingerprinting of serratiopeptidase, obtained from it. In order to obtain high purity of serratiopeptidase from the fermentation broth of pure culture isolate of Serratia marcescens, some separation and purification techniques were also explored.  The results demonstrate that the combination of bio separation   processes of ammonium sulfate precipitation with ion exchange chromatography is suitable for the separation and purification of serratiopeptidase from culture broth of Serratia marcescens. Peptide mass finger printing was performed and the amino acid sequence of serratiopeptidase was elucidated, compared and confirmed with clustal pair wise alignment database tool to confirm that the product produced and purified was serratiopeptidase from Serratia marcescens. Thus, indirectly Serratia marcescens has been completely genetically characterized using its therapeutically important product serratiopeptidase and the 16s rRNA sequencing of the bacteria.