Phytochemistry And Antibacterial Activity Test From Methanol Extract of Alstonia Acuminata Tree Bark Against Vibrio Harveyi Bacterium in In-Vitro Manner

Abstract

Grouper belongs to one of cultivated commodities whose high selling price gives bright prospects.Grouper belongs to one of cultivated commodities whose high selling price gives bright prospects. However, the greatest faced obstacle in this aquaculture industry lies in disease strike brought by bacterium. Synthetic antibiotic use made from chemical compounds gives long-term negative effect and accumulates the chemical compounds into body tissues. Natural material use as an antibacterial is highly recommended to reduce side effect of medicinal effect. The use of antibacterial extract made from A. acuminata tree bark will give new discourse in handling mischievously bacterial strike problem. The study aims to find out chemical compounds made from A. acuminata and to find out solvent and the best dose of A. acuminata inhibition activity as antibacterial against Vibrio harveyi bacterium. Based on research findings, it is known that A. acuminata extract with methanol solvent has the highest inhibition activity compared to ethyl acetate and n-hexane solvent. Then, a concentration of 100% has the highest inhibition activity compared to concentration of 15%, 25%, 50% and 75%. Phytochemistry test result shows that A. acuminata tree bark contains compounds of alkaloid, phenol, flavonoid, steroid, and terpenoids. Based on the findings, it is concluded that A. acuminata contains phenol compound which is able to increase inhibition activity against V. harveyi bacterium.