Phenotypic-Genotypic Characterization and Enzyme Activity of Bacillus Spp. Isolated from Saricay Stream

Abstract

63 bacteria consist of Bacillus genus were isolated from water samples of Saricay stream (Canakkale). Isolates were characterized for phenotypic properties, antimicrobial and heavy metal resistance and evaluated for some extracellular enzyme activities. 13 bacterial colonies were selected according to their different phenotypic-genotypic characteristics and described by 16S rDNA sequence analysis methods.

There was a high incidence of resistance to Cefoxitin (CN30 μg/mL), while all of isolates sensitive against to Kanamycin (K30 μg/mL) and Chloramphenicol (C30 μg/mL). The trends in heavy metal resistance: Chromium (Cr) > (Zinc ) Zn > Copper (Cu) > Manganese (Mn). Totally 55 protease, 49 amylase, 30 cellulase and 59 lipase activities were determined from all of isolated Bacillus sp. None of the isolates showed the esterase activity. As a result of molecular identification six isolates were showed the highest similarity to different strain of Bacillus amyloliquefaciens and others similar to Bacillus sp. B22(2008), B. subtilis SD-45, Sporolactobacillus dextrus, Bacillus sp. IHBB3375, B. cereus RJ1, Uncultured organism clone ELU0176-T465-S-NIPCRAMgANa_000604, Uncultured organism clone ELU0110-T273-S-NI_000292.

This study is first of its kind to explore the bacterial enzyme resource from Saricay stream. This work forms the foundation for future research to enzyme characterization and assessed using in industrial fields explores the biotechnological potential of these Bacillus sp. isolates.