Depletion of CD45 as Pre-Enrichment Step for Rare Cells Detection Using a Novel Immunomagnetic Technology

Abstract

Isolation of rare cells from peripheral blood may be helpful either in the detection of circulating tumor cells and directing aggressiveness of therapy, or it can be helpful for prenatal diagnosis by the enrichment of fetal cells from maternal blood. The major limit of detection rare cells using magnetic beads is primarily influenced by the non-specific binding of non-target cells. The depletion of non-target cells is considered an important prerequisite for rare cells detection.

In this study, we examined and compared the depletion efficiency of CD45 cell from peripheral blood samples using two different forms of immunomagnetic separation technology namely: the Cell enrichment process (CEPir) and DynaMAG technology.

Our results indicate significantly lower number of CD45 cells in negative fractions using the CEPir technology. Moreover, the CD45 depletion efficiency was found to be higher in CEPir technology when compared with the DynaMAG.

Negative selection is a promising approach for isolation of rare cells, we concluded that the negative selection (depletion) of leukocytes using CD45 marker is an effective pre-enrichment step for the detection of rare cells using CEPir technology. Further studies are required to validate its efficacy at capturing specific rare cells for downstream application.