Preventive Effect of Tephrosia Purpurea Against N,N-Diethylnitrosamine Induced Hepatocellular Carcinoma In Swiss Albino Mice

Abstract

The aim of the present study is to divulge the preventive nature of Tephrosia purpurea Methanolic Extract (TpME) during DiEthylNitrosAmine (DENA) induced liver cancer in male Swiss albino mice. Anticancer activity of methanolic extract of Tephrosia purpurea was evaluated in DENA induced Swiss albino mice at the doses of 200 and 300 mg/kg body weight orally and both doses were administered for 14 days. After 24 h of the last dose, the mice were sacrificed and antitumor effect of TpME was assessed by evaluating liver serum marker enzyme and haematological parameters. Decrease in Aspartate transaminase, Alanine transaminase, Alkaline phosphatase, Bilirubin and Total protein count were observed in TpME treated animals when compared to DENA treated animals. Haematological profiles such as haemoglobin, red blood cell (RBC) and white blood cell (WBC) count reverted to normal level in TpME treated mice. A comparative histopathology study of liver from test group exhibited almost normal architecture, as compared to DENA treated group. The results demonstrated that Tephrosia purpurea methanolic extract at 300 mg/kg dose exhibits significant antitumor activity than 200 mg/kg.