Rapid Diagnosis of Highly Pathogenic Clinical Strains of Yersinia Pseudotuberculosis Using PCR and Preservation of 70Kbp Virulence Plasmid

Abstract

Clinical strains of Yersinia.pseudotuberculosis were screened for 70 Kbp virulence plasmids it was found only 20 % carrying 70Kbp plasmid, when subjected to different concentrations of Naledixin for development of mutant strains ,  plasmids were lost from one strain Yersinia pseudotuberculosis IP3295, which leaded to the modification of L.B medium into human blood (H.B) L.B medium as culture medium for Yersinia pseudotuberculosis .Yersinia pseudotuberculosis IP3295 showed 70Kbp virulence plasmid with additional small plasmids which proves the incorporation of plasmids with chromosomal DNA and similarity with Yersinia pestis. All strains of Yersinia pseudotuberculosis harboring virulence plasmids were detected for FyuA and Irp2 genes of the High pathogenicity island using PCR. And it was found that all plasmid harboring strains contains high Pathogenicity Island.